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pvegfr2 tyr951 cell signaling 2471 s wb rabbit  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pvegfr2 tyr951 cell signaling 2471 s wb rabbit
    Pvegfr2 Tyr951 Cell Signaling 2471 S Wb Rabbit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s+wb/Phospho-VEGF+Receptor+2+(Tyr951)+Antibody/pmc12453065__iovs-66-12-45_s001-42-104-106
    Average 94 stars, based on 129 article reviews
    pvegfr2 tyr951 cell signaling 2471 s wb rabbit - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) CRACR2A NCBI Gene ID: 84,766 Strain, strain background (Escherichia coli) DH5α Thermo fisher Scientific Cat# 18265017 Cell line (Homo sapiens) Jurkat E6- 1 T cells ATCC Not tested for mycoplasma Cat# TIB- 152 Cell line (Homo sapiens) HEK293T ATCC Not tested for mycoplasma Cat# CRL- 3216 Cell line (Mus musculus) CD40L- expressing L cell fibroblasts Diehl et al., 2008 Irradiated at 50 Gy for 50 minutes prior to use Cell line (Mus musculus) M2- 10B4 bone marrow stromal cells Lemoine et al., 1988 Irradiated at 57 Gy for 57 minutes prior to use Transfected construct (Homo sapiens) Primers for plasmid construction This paper Supplementary file 1 Biological sample (Homo- sapiens) Primary human peripheral blood mononuclear cells This paper Isolated from the patient and healthy controls Antibody Anti- human Phospho JNK (mouse monoclonal) Cell Signaling Technologies Cat# 9,255 Flow cytometry (1:100) Antibody Anti- Human STIM1(rabbit) Cell Signaling Technologies Cat# 5,668 S WB (1:5000) Antibody Anti- His- tag (rabbit) Cell Signaling Technologies Cat# 12,698 S WB (1:5000) Antibody Anti- FLAG tag (mouse monoclonal) Millipore Sigma Cat# F3040 WB (1:5000) Antibody Anti- human ORAI1 (rabbit polyclonal) Millipore Sigma Cat# AB9868 WB (1:5000) Antibody Anti- Vav1 (rabbit) Cell Signaling Technologies Cat# 2,502 WB (1:2000) Antibody Anti-β-actin (mouse monoclonal) Santa Cruz Biotechnology Cat# sc- 47778 WB (1:2500) Antibody anti- human CD3 antibody (mouse monoclonal) Bio X Cell Clone OKT- 3 1 μg/ml Antibody Anti- human CD28 antibody (mouse monoclonal) Bio X Cell Cat. #: BE0921 Clone CD28.2 1 μg/ml Antibody Anti- CD4- FITC (mouse monoclonal) eBioscience Clone OKT- 4 FACS 5 μl/test Antibody Anti- IFNgama- PE- Cy7 eBioscience Clone 45.B3 FACS 5 μl/test Antibody Ant- IL2- PE eBioscience Cat# MQ1- 17H12 FACS 5 μl/test Wu, Rice, et al. eLife 2021;0:e72559. ..

    Irradiation:

    Article Title: Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) CRACR2A NCBI Gene ID: 84,766 Strain, strain background (Escherichia coli) DH5α Thermo fisher Scientific Cat# 18265017 Cell line (Homo sapiens) Jurkat E6- 1 T cells ATCC Not tested for mycoplasma Cat# TIB- 152 Cell line (Homo sapiens) HEK293T ATCC Not tested for mycoplasma Cat# CRL- 3216 Cell line (Mus musculus) CD40L- expressing L cell fibroblasts Diehl et al., 2008 Irradiated at 50 Gy for 50 minutes prior to use Cell line (Mus musculus) M2- 10B4 bone marrow stromal cells Lemoine et al., 1988 Irradiated at 57 Gy for 57 minutes prior to use Transfected construct (Homo sapiens) Primers for plasmid construction This paper Supplementary file 1 Biological sample (Homo- sapiens) Primary human peripheral blood mononuclear cells This paper Isolated from the patient and healthy controls Antibody Anti- human Phospho JNK (mouse monoclonal) Cell Signaling Technologies Cat# 9,255 Flow cytometry (1:100) Antibody Anti- Human STIM1(rabbit) Cell Signaling Technologies Cat# 5,668 S WB (1:5000) Antibody Anti- His- tag (rabbit) Cell Signaling Technologies Cat# 12,698 S WB (1:5000) Antibody Anti- FLAG tag (mouse monoclonal) Millipore Sigma Cat# F3040 WB (1:5000) Antibody Anti- human ORAI1 (rabbit polyclonal) Millipore Sigma Cat# AB9868 WB (1:5000) Antibody Anti- Vav1 (rabbit) Cell Signaling Technologies Cat# 2,502 WB (1:2000) Antibody Anti-β-actin (mouse monoclonal) Santa Cruz Biotechnology Cat# sc- 47778 WB (1:2500) Antibody anti- human CD3 antibody (mouse monoclonal) Bio X Cell Clone OKT- 3 1 μg/ml Antibody Anti- human CD28 antibody (mouse monoclonal) Bio X Cell Cat. #: BE0921 Clone CD28.2 1 μg/ml Antibody Anti- CD4- FITC (mouse monoclonal) eBioscience Clone OKT- 4 FACS 5 μl/test Antibody Anti- IFNgama- PE- Cy7 eBioscience Clone 45.B3 FACS 5 μl/test Antibody Ant- IL2- PE eBioscience Cat# MQ1- 17H12 FACS 5 μl/test Wu, Rice, et al. eLife 2021;0:e72559. ..

    Transfection:

    Article Title: Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) CRACR2A NCBI Gene ID: 84,766 Strain, strain background (Escherichia coli) DH5α Thermo fisher Scientific Cat# 18265017 Cell line (Homo sapiens) Jurkat E6- 1 T cells ATCC Not tested for mycoplasma Cat# TIB- 152 Cell line (Homo sapiens) HEK293T ATCC Not tested for mycoplasma Cat# CRL- 3216 Cell line (Mus musculus) CD40L- expressing L cell fibroblasts Diehl et al., 2008 Irradiated at 50 Gy for 50 minutes prior to use Cell line (Mus musculus) M2- 10B4 bone marrow stromal cells Lemoine et al., 1988 Irradiated at 57 Gy for 57 minutes prior to use Transfected construct (Homo sapiens) Primers for plasmid construction This paper Supplementary file 1 Biological sample (Homo- sapiens) Primary human peripheral blood mononuclear cells This paper Isolated from the patient and healthy controls Antibody Anti- human Phospho JNK (mouse monoclonal) Cell Signaling Technologies Cat# 9,255 Flow cytometry (1:100) Antibody Anti- Human STIM1(rabbit) Cell Signaling Technologies Cat# 5,668 S WB (1:5000) Antibody Anti- His- tag (rabbit) Cell Signaling Technologies Cat# 12,698 S WB (1:5000) Antibody Anti- FLAG tag (mouse monoclonal) Millipore Sigma Cat# F3040 WB (1:5000) Antibody Anti- human ORAI1 (rabbit polyclonal) Millipore Sigma Cat# AB9868 WB (1:5000) Antibody Anti- Vav1 (rabbit) Cell Signaling Technologies Cat# 2,502 WB (1:2000) Antibody Anti-β-actin (mouse monoclonal) Santa Cruz Biotechnology Cat# sc- 47778 WB (1:2500) Antibody anti- human CD3 antibody (mouse monoclonal) Bio X Cell Clone OKT- 3 1 μg/ml Antibody Anti- human CD28 antibody (mouse monoclonal) Bio X Cell Cat. #: BE0921 Clone CD28.2 1 μg/ml Antibody Anti- CD4- FITC (mouse monoclonal) eBioscience Clone OKT- 4 FACS 5 μl/test Antibody Anti- IFNgama- PE- Cy7 eBioscience Clone 45.B3 FACS 5 μl/test Antibody Ant- IL2- PE eBioscience Cat# MQ1- 17H12 FACS 5 μl/test Wu, Rice, et al. eLife 2021;0:e72559. ..

    Construct:

    Article Title: Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) CRACR2A NCBI Gene ID: 84,766 Strain, strain background (Escherichia coli) DH5α Thermo fisher Scientific Cat# 18265017 Cell line (Homo sapiens) Jurkat E6- 1 T cells ATCC Not tested for mycoplasma Cat# TIB- 152 Cell line (Homo sapiens) HEK293T ATCC Not tested for mycoplasma Cat# CRL- 3216 Cell line (Mus musculus) CD40L- expressing L cell fibroblasts Diehl et al., 2008 Irradiated at 50 Gy for 50 minutes prior to use Cell line (Mus musculus) M2- 10B4 bone marrow stromal cells Lemoine et al., 1988 Irradiated at 57 Gy for 57 minutes prior to use Transfected construct (Homo sapiens) Primers for plasmid construction This paper Supplementary file 1 Biological sample (Homo- sapiens) Primary human peripheral blood mononuclear cells This paper Isolated from the patient and healthy controls Antibody Anti- human Phospho JNK (mouse monoclonal) Cell Signaling Technologies Cat# 9,255 Flow cytometry (1:100) Antibody Anti- Human STIM1(rabbit) Cell Signaling Technologies Cat# 5,668 S WB (1:5000) Antibody Anti- His- tag (rabbit) Cell Signaling Technologies Cat# 12,698 S WB (1:5000) Antibody Anti- FLAG tag (mouse monoclonal) Millipore Sigma Cat# F3040 WB (1:5000) Antibody Anti- human ORAI1 (rabbit polyclonal) Millipore Sigma Cat# AB9868 WB (1:5000) Antibody Anti- Vav1 (rabbit) Cell Signaling Technologies Cat# 2,502 WB (1:2000) Antibody Anti-β-actin (mouse monoclonal) Santa Cruz Biotechnology Cat# sc- 47778 WB (1:2500) Antibody anti- human CD3 antibody (mouse monoclonal) Bio X Cell Clone OKT- 3 1 μg/ml Antibody Anti- human CD28 antibody (mouse monoclonal) Bio X Cell Cat. #: BE0921 Clone CD28.2 1 μg/ml Antibody Anti- CD4- FITC (mouse monoclonal) eBioscience Clone OKT- 4 FACS 5 μl/test Antibody Anti- IFNgama- PE- Cy7 eBioscience Clone 45.B3 FACS 5 μl/test Antibody Ant- IL2- PE eBioscience Cat# MQ1- 17H12 FACS 5 μl/test Wu, Rice, et al. eLife 2021;0:e72559. ..

    Plasmid Preparation:

    Article Title: Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) CRACR2A NCBI Gene ID: 84,766 Strain, strain background (Escherichia coli) DH5α Thermo fisher Scientific Cat# 18265017 Cell line (Homo sapiens) Jurkat E6- 1 T cells ATCC Not tested for mycoplasma Cat# TIB- 152 Cell line (Homo sapiens) HEK293T ATCC Not tested for mycoplasma Cat# CRL- 3216 Cell line (Mus musculus) CD40L- expressing L cell fibroblasts Diehl et al., 2008 Irradiated at 50 Gy for 50 minutes prior to use Cell line (Mus musculus) M2- 10B4 bone marrow stromal cells Lemoine et al., 1988 Irradiated at 57 Gy for 57 minutes prior to use Transfected construct (Homo sapiens) Primers for plasmid construction This paper Supplementary file 1 Biological sample (Homo- sapiens) Primary human peripheral blood mononuclear cells This paper Isolated from the patient and healthy controls Antibody Anti- human Phospho JNK (mouse monoclonal) Cell Signaling Technologies Cat# 9,255 Flow cytometry (1:100) Antibody Anti- Human STIM1(rabbit) Cell Signaling Technologies Cat# 5,668 S WB (1:5000) Antibody Anti- His- tag (rabbit) Cell Signaling Technologies Cat# 12,698 S WB (1:5000) Antibody Anti- FLAG tag (mouse monoclonal) Millipore Sigma Cat# F3040 WB (1:5000) Antibody Anti- human ORAI1 (rabbit polyclonal) Millipore Sigma Cat# AB9868 WB (1:5000) Antibody Anti- Vav1 (rabbit) Cell Signaling Technologies Cat# 2,502 WB (1:2000) Antibody Anti-β-actin (mouse monoclonal) Santa Cruz Biotechnology Cat# sc- 47778 WB (1:2500) Antibody anti- human CD3 antibody (mouse monoclonal) Bio X Cell Clone OKT- 3 1 μg/ml Antibody Anti- human CD28 antibody (mouse monoclonal) Bio X Cell Cat. #: BE0921 Clone CD28.2 1 μg/ml Antibody Anti- CD4- FITC (mouse monoclonal) eBioscience Clone OKT- 4 FACS 5 μl/test Antibody Anti- IFNgama- PE- Cy7 eBioscience Clone 45.B3 FACS 5 μl/test Antibody Ant- IL2- PE eBioscience Cat# MQ1- 17H12 FACS 5 μl/test Wu, Rice, et al. eLife 2021;0:e72559. ..

    Isolation:

    Article Title: Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) CRACR2A NCBI Gene ID: 84,766 Strain, strain background (Escherichia coli) DH5α Thermo fisher Scientific Cat# 18265017 Cell line (Homo sapiens) Jurkat E6- 1 T cells ATCC Not tested for mycoplasma Cat# TIB- 152 Cell line (Homo sapiens) HEK293T ATCC Not tested for mycoplasma Cat# CRL- 3216 Cell line (Mus musculus) CD40L- expressing L cell fibroblasts Diehl et al., 2008 Irradiated at 50 Gy for 50 minutes prior to use Cell line (Mus musculus) M2- 10B4 bone marrow stromal cells Lemoine et al., 1988 Irradiated at 57 Gy for 57 minutes prior to use Transfected construct (Homo sapiens) Primers for plasmid construction This paper Supplementary file 1 Biological sample (Homo- sapiens) Primary human peripheral blood mononuclear cells This paper Isolated from the patient and healthy controls Antibody Anti- human Phospho JNK (mouse monoclonal) Cell Signaling Technologies Cat# 9,255 Flow cytometry (1:100) Antibody Anti- Human STIM1(rabbit) Cell Signaling Technologies Cat# 5,668 S WB (1:5000) Antibody Anti- His- tag (rabbit) Cell Signaling Technologies Cat# 12,698 S WB (1:5000) Antibody Anti- FLAG tag (mouse monoclonal) Millipore Sigma Cat# F3040 WB (1:5000) Antibody Anti- human ORAI1 (rabbit polyclonal) Millipore Sigma Cat# AB9868 WB (1:5000) Antibody Anti- Vav1 (rabbit) Cell Signaling Technologies Cat# 2,502 WB (1:2000) Antibody Anti-β-actin (mouse monoclonal) Santa Cruz Biotechnology Cat# sc- 47778 WB (1:2500) Antibody anti- human CD3 antibody (mouse monoclonal) Bio X Cell Clone OKT- 3 1 μg/ml Antibody Anti- human CD28 antibody (mouse monoclonal) Bio X Cell Cat. #: BE0921 Clone CD28.2 1 μg/ml Antibody Anti- CD4- FITC (mouse monoclonal) eBioscience Clone OKT- 4 FACS 5 μl/test Antibody Anti- IFNgama- PE- Cy7 eBioscience Clone 45.B3 FACS 5 μl/test Antibody Ant- IL2- PE eBioscience Cat# MQ1- 17H12 FACS 5 μl/test Wu, Rice, et al. eLife 2021;0:e72559. ..

    Flow Cytometry:

    Article Title: Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) CRACR2A NCBI Gene ID: 84,766 Strain, strain background (Escherichia coli) DH5α Thermo fisher Scientific Cat# 18265017 Cell line (Homo sapiens) Jurkat E6- 1 T cells ATCC Not tested for mycoplasma Cat# TIB- 152 Cell line (Homo sapiens) HEK293T ATCC Not tested for mycoplasma Cat# CRL- 3216 Cell line (Mus musculus) CD40L- expressing L cell fibroblasts Diehl et al., 2008 Irradiated at 50 Gy for 50 minutes prior to use Cell line (Mus musculus) M2- 10B4 bone marrow stromal cells Lemoine et al., 1988 Irradiated at 57 Gy for 57 minutes prior to use Transfected construct (Homo sapiens) Primers for plasmid construction This paper Supplementary file 1 Biological sample (Homo- sapiens) Primary human peripheral blood mononuclear cells This paper Isolated from the patient and healthy controls Antibody Anti- human Phospho JNK (mouse monoclonal) Cell Signaling Technologies Cat# 9,255 Flow cytometry (1:100) Antibody Anti- Human STIM1(rabbit) Cell Signaling Technologies Cat# 5,668 S WB (1:5000) Antibody Anti- His- tag (rabbit) Cell Signaling Technologies Cat# 12,698 S WB (1:5000) Antibody Anti- FLAG tag (mouse monoclonal) Millipore Sigma Cat# F3040 WB (1:5000) Antibody Anti- human ORAI1 (rabbit polyclonal) Millipore Sigma Cat# AB9868 WB (1:5000) Antibody Anti- Vav1 (rabbit) Cell Signaling Technologies Cat# 2,502 WB (1:2000) Antibody Anti-β-actin (mouse monoclonal) Santa Cruz Biotechnology Cat# sc- 47778 WB (1:2500) Antibody anti- human CD3 antibody (mouse monoclonal) Bio X Cell Clone OKT- 3 1 μg/ml Antibody Anti- human CD28 antibody (mouse monoclonal) Bio X Cell Cat. #: BE0921 Clone CD28.2 1 μg/ml Antibody Anti- CD4- FITC (mouse monoclonal) eBioscience Clone OKT- 4 FACS 5 μl/test Antibody Anti- IFNgama- PE- Cy7 eBioscience Clone 45.B3 FACS 5 μl/test Antibody Ant- IL2- PE eBioscience Cat# MQ1- 17H12 FACS 5 μl/test Wu, Rice, et al. eLife 2021;0:e72559. ..

    Western Blot:

    Article Title: Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) CRACR2A NCBI Gene ID: 84,766 Strain, strain background (Escherichia coli) DH5α Thermo fisher Scientific Cat# 18265017 Cell line (Homo sapiens) Jurkat E6- 1 T cells ATCC Not tested for mycoplasma Cat# TIB- 152 Cell line (Homo sapiens) HEK293T ATCC Not tested for mycoplasma Cat# CRL- 3216 Cell line (Mus musculus) CD40L- expressing L cell fibroblasts Diehl et al., 2008 Irradiated at 50 Gy for 50 minutes prior to use Cell line (Mus musculus) M2- 10B4 bone marrow stromal cells Lemoine et al., 1988 Irradiated at 57 Gy for 57 minutes prior to use Transfected construct (Homo sapiens) Primers for plasmid construction This paper Supplementary file 1 Biological sample (Homo- sapiens) Primary human peripheral blood mononuclear cells This paper Isolated from the patient and healthy controls Antibody Anti- human Phospho JNK (mouse monoclonal) Cell Signaling Technologies Cat# 9,255 Flow cytometry (1:100) Antibody Anti- Human STIM1(rabbit) Cell Signaling Technologies Cat# 5,668 S WB (1:5000) Antibody Anti- His- tag (rabbit) Cell Signaling Technologies Cat# 12,698 S WB (1:5000) Antibody Anti- FLAG tag (mouse monoclonal) Millipore Sigma Cat# F3040 WB (1:5000) Antibody Anti- human ORAI1 (rabbit polyclonal) Millipore Sigma Cat# AB9868 WB (1:5000) Antibody Anti- Vav1 (rabbit) Cell Signaling Technologies Cat# 2,502 WB (1:2000) Antibody Anti-β-actin (mouse monoclonal) Santa Cruz Biotechnology Cat# sc- 47778 WB (1:2500) Antibody anti- human CD3 antibody (mouse monoclonal) Bio X Cell Clone OKT- 3 1 μg/ml Antibody Anti- human CD28 antibody (mouse monoclonal) Bio X Cell Cat. #: BE0921 Clone CD28.2 1 μg/ml Antibody Anti- CD4- FITC (mouse monoclonal) eBioscience Clone OKT- 4 FACS 5 μl/test Antibody Anti- IFNgama- PE- Cy7 eBioscience Clone 45.B3 FACS 5 μl/test Antibody Ant- IL2- PE eBioscience Cat# MQ1- 17H12 FACS 5 μl/test Wu, Rice, et al. eLife 2021;0:e72559. ..

    FLAG-tag:

    Article Title: Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) CRACR2A NCBI Gene ID: 84,766 Strain, strain background (Escherichia coli) DH5α Thermo fisher Scientific Cat# 18265017 Cell line (Homo sapiens) Jurkat E6- 1 T cells ATCC Not tested for mycoplasma Cat# TIB- 152 Cell line (Homo sapiens) HEK293T ATCC Not tested for mycoplasma Cat# CRL- 3216 Cell line (Mus musculus) CD40L- expressing L cell fibroblasts Diehl et al., 2008 Irradiated at 50 Gy for 50 minutes prior to use Cell line (Mus musculus) M2- 10B4 bone marrow stromal cells Lemoine et al., 1988 Irradiated at 57 Gy for 57 minutes prior to use Transfected construct (Homo sapiens) Primers for plasmid construction This paper Supplementary file 1 Biological sample (Homo- sapiens) Primary human peripheral blood mononuclear cells This paper Isolated from the patient and healthy controls Antibody Anti- human Phospho JNK (mouse monoclonal) Cell Signaling Technologies Cat# 9,255 Flow cytometry (1:100) Antibody Anti- Human STIM1(rabbit) Cell Signaling Technologies Cat# 5,668 S WB (1:5000) Antibody Anti- His- tag (rabbit) Cell Signaling Technologies Cat# 12,698 S WB (1:5000) Antibody Anti- FLAG tag (mouse monoclonal) Millipore Sigma Cat# F3040 WB (1:5000) Antibody Anti- human ORAI1 (rabbit polyclonal) Millipore Sigma Cat# AB9868 WB (1:5000) Antibody Anti- Vav1 (rabbit) Cell Signaling Technologies Cat# 2,502 WB (1:2000) Antibody Anti-β-actin (mouse monoclonal) Santa Cruz Biotechnology Cat# sc- 47778 WB (1:2500) Antibody anti- human CD3 antibody (mouse monoclonal) Bio X Cell Clone OKT- 3 1 μg/ml Antibody Anti- human CD28 antibody (mouse monoclonal) Bio X Cell Cat. #: BE0921 Clone CD28.2 1 μg/ml Antibody Anti- CD4- FITC (mouse monoclonal) eBioscience Clone OKT- 4 FACS 5 μl/test Antibody Anti- IFNgama- PE- Cy7 eBioscience Clone 45.B3 FACS 5 μl/test Antibody Ant- IL2- PE eBioscience Cat# MQ1- 17H12 FACS 5 μl/test Wu, Rice, et al. eLife 2021;0:e72559. ..

    FACS:

    Article Title: Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) CRACR2A NCBI Gene ID: 84,766 Strain, strain background (Escherichia coli) DH5α Thermo fisher Scientific Cat# 18265017 Cell line (Homo sapiens) Jurkat E6- 1 T cells ATCC Not tested for mycoplasma Cat# TIB- 152 Cell line (Homo sapiens) HEK293T ATCC Not tested for mycoplasma Cat# CRL- 3216 Cell line (Mus musculus) CD40L- expressing L cell fibroblasts Diehl et al., 2008 Irradiated at 50 Gy for 50 minutes prior to use Cell line (Mus musculus) M2- 10B4 bone marrow stromal cells Lemoine et al., 1988 Irradiated at 57 Gy for 57 minutes prior to use Transfected construct (Homo sapiens) Primers for plasmid construction This paper Supplementary file 1 Biological sample (Homo- sapiens) Primary human peripheral blood mononuclear cells This paper Isolated from the patient and healthy controls Antibody Anti- human Phospho JNK (mouse monoclonal) Cell Signaling Technologies Cat# 9,255 Flow cytometry (1:100) Antibody Anti- Human STIM1(rabbit) Cell Signaling Technologies Cat# 5,668 S WB (1:5000) Antibody Anti- His- tag (rabbit) Cell Signaling Technologies Cat# 12,698 S WB (1:5000) Antibody Anti- FLAG tag (mouse monoclonal) Millipore Sigma Cat# F3040 WB (1:5000) Antibody Anti- human ORAI1 (rabbit polyclonal) Millipore Sigma Cat# AB9868 WB (1:5000) Antibody Anti- Vav1 (rabbit) Cell Signaling Technologies Cat# 2,502 WB (1:2000) Antibody Anti-β-actin (mouse monoclonal) Santa Cruz Biotechnology Cat# sc- 47778 WB (1:2500) Antibody anti- human CD3 antibody (mouse monoclonal) Bio X Cell Clone OKT- 3 1 μg/ml Antibody Anti- human CD28 antibody (mouse monoclonal) Bio X Cell Cat. #: BE0921 Clone CD28.2 1 μg/ml Antibody Anti- CD4- FITC (mouse monoclonal) eBioscience Clone OKT- 4 FACS 5 μl/test Antibody Anti- IFNgama- PE- Cy7 eBioscience Clone 45.B3 FACS 5 μl/test Antibody Ant- IL2- PE eBioscience Cat# MQ1- 17H12 FACS 5 μl/test Wu, Rice, et al. eLife 2021;0:e72559. ..



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    The index tests (LFAs) used in this study (all used a cassette platform).

    Journal: PLOS Neglected Tropical Diseases

    Article Title: Comparative evaluation of lateral flow assays to diagnose chronic Trypanosoma cruzi infection in Bolivia

    doi: 10.1371/journal.pntd.0012016

    Figure Lengend Snippet: The index tests (LFAs) used in this study (all used a cassette platform).

    Article Snippet: Their specificities were all significantly higher than those of the Chagas Rapid Test Cassette WB/S/P (Acro Biotech, USA) (p < 2.25e-3) and TR Chagas—Biomanguinhos (Biomanguinhos, Brazil) (p < 8.64e-8).

    Techniques: Recombinant, Diagnostic Assay